• The objectives of this study were to

  2024-09-14

  

  The objectives of this study were to investigate 1) the mRNA and protein expression of apelin and APJ in porcine ovarian follicles of different sizes, and their immunolocalization and concentrations in follicular fluid and ovarian follicle, and 2) the direct effect of recombinant human apelin-13 on

• br Acknowledgments br Protein microarrays were developed to

  2024-09-14

  

  Acknowledgments Protein microarrays were developed to provide miniaturized high-throughput tools to study protein function, Phosphatase Inhibitor Cocktail 1 (100X in DMSO) and post-translational modifications. Just a few years ago, the seminal work performed in Stuart Schreiber's laboratory at

• We made a number of choices in

  2024-09-14

  

  We made a number of choices in the original design of our experiments. First, we used only portions of the 5′ and 3′ regulatory elements from S. cerevisiae. The sequences of these regions were known [32] and our goal was to express the Pf-DHFR at low levels. In fact, our choice of only 600 bp of the

• The reactions and the control mechanisms described

  2024-09-14

  

  The reactions and the control mechanisms described are all supported by observations. For example the reactions require that different compounds are formed in different groups of exportin cells and this has been observed [15]. Intravenous injection of glycine results in reduced utilisation of gluco

• br Results and discussion The

  2024-09-14

  

  Results and discussion The classical radiochemical adenosine kinase assays developed to monitor AdK activity utilize [3H]adenosine or [14C]adenosine, respectively. The assays are terminated by spotting the incubation mixture onto diethylaminoethyl (DEAE) anion exchange filter disks or onto a poly

• TH-302 receptor Western blot results showed that the express

  2024-09-13

  

  Western blot results showed that the expressions of SOCS1 and SOCS3 significantly increased after administration of rGas6 24h after MCAO (Fig. 7A–C p Discussion After ischemic stroke, inflammation has been shown to be an important secondary injury mediator in patients, as well as in animals (Herz

• To help ensure the fidelity of mitosis and cytokinesis cells

  2024-09-13

  

  To help ensure the fidelity of mitosis and cytokinesis cells monitor these processes and delay cell cycle progression in response to certain defects. The spindle assembly checkpoint (SAC) is a surveillance system which delays s6 kinase onset until a bipolar spindle has been correctly assembled (Lar

• Purification of Rv c In order

  2024-09-13

  

  Purification of Rv2477c. In order to investigate the catalytic capabilities of Rv2477c, we expressed the protein as an N-terminal histidine-tagged fusion protein in E. coli BL21 cells and purified the protein by cobalt-affinity chromatography. As shown in Fig. 2A, the 65 kDa fusion protein was expre

• The apoptosis promoting Bcl family

  2024-09-13

  

  The apoptosis-promoting Bcl-2 family includes BH3-only proteins (e.g., Bim, Bid and Bad) and multiple-domain proteins (e.g., Bax and Bak). At the time of apoptosis stimulation (e.g., DNA damage), only the BH3-only protein is activated, directly or indirectly promoting Bax/Bak oligomerization, changi

• br Materials and methods br Results

  2024-09-13

  

  Materials and methods Results and discussion During recent years, recombinant ASK1 has been obtained by expensive baculovirus-insect cell RGDS peptide receptor systems [20], [27]. In the present study, we constructed recombinant ASK1 and expressed in active form at high level in E. coli. Huma

• A different mechanism has been suggested for the

  2024-09-13

  

  A different mechanism has been suggested for the role of TRX1 in ASK1 regulation based on disulfide bond-mediated ASK1 multimerization and its reduction through the thiol-reductase activity of TRX1 (Nadeau et al., 2007, Nadeau et al., 2009). In this model, oxidative stress induces intermolecular dis

• ARG I is one of the most important mammalian

  2024-09-13

  

  ARG I is one of the most important mammalian enzymes responsible for nitrogen metabolism since it comprises the main route for the elimination of excess nitrogen resulting from amino Protease Inhibitor Library and nucleotide metabolism [18]. ARG I deficiency leads to hyperargininemia, characterized

• br Asthma The chronic airway inflammatory disease asthma

  2024-09-13

  

  Asthma The chronic airway inflammatory disease cyclic adenosine monophosphate is associated with enhanced levels of exhaled NO generated by iNOS in the airway epithelium [12]. In asthmatic patients local and systemic changes in iNOS, peroxynitrite, arginase, ADMA and arginine levels have been ob

• ACP-196 australia br Experimental section br Results and dis

  2024-09-13

  

  Experimental section Results and discussion Conclusion Acknowledgement This work was supported by the National Natural Science Foundation of China (Grant Nos. 81401489), the Shanghai Pujiang Program (17PJ1402800) and the Shanghai Sailing Program (Grant No. 14YF1409000). Introduction

• Introduction Aldose reductase ALR is

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  Introduction Aldose reductase (ALR2) is the first enzyme of the polyol pathway that catalyzes the reduction of glucose to sorbitol utilizing NADPH as a cofactor. The intracellular accumulation of sorbitol, due to increased aldose reductase activity at high blood glucose levels, such as those occurr

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